Western blot hiv principle11/21/2023 ![]() Western blotting is also used to test for phosphorylation signal, structure domain analysis and analysis of protein express level. In disease diagnostics, Western blotting can be used to test for HIV by detecting the HIV antibody in the serum. Western blotting can have a number of applications in sciences ranging from determination of size and amount of protein in given sample to test for some diseases like the Lyme disease. Polyacrylamide gel electrophoresis (PAGE) is used for separating proteins ranging in size from 5 to 2,000 kDa. Polyacrylamide gel (PAG) possesses several desirable features that make it a versatile medium. The principle based on the fact that charged particles move towards the opposite electrode when current is applied this separates the proteins according to mobility which depends on size, charge and structure of the protein. Western blotting technique is used in fields such as molecular biology, biochemistry and immunogenetics. ![]() In this technique SDS-PAGE (SDS- polyacrelamide gel electrophoresis) is used to differentiate or separate the protein present on the sample either by structure or length of polypeptide. Western blotting is an analytical process or technique that is used to detect protein from a given sample it is also sometimes referred to as protein immunoblotting due to the fact that it involves use of an antibody to detect the presence of an antigen. ![]()
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